Lopez, O'Reilly, Klimov

Centrifugal elutriation - pre-sort particles

Sampling statistics problem

Partec - flow microscope

Denis - showed SM data
PL: Could you acoustically control concentration in SM measurement volume, to prevent excessive particles?

Round table:

Influx sorting discussion with Camille

Scholin: interested in particle-field distribution, to guide adaptive sampling
CS: Which is more amenable to real-time sampling: scatter/fluor vs morphological?
PL: Need to decide size range. Answer is not always flow cytometry. Look at Shapiro's battery-powered microscope, for remote diagnostics

JB: How small can a flow cytometer be?
PL: lasers are really small now... a lot of cytometers had to be flexible - now more specialized, smaller versions available. HIV/AIDS applications in developing world are driving size/power downward.
CS: What are prospects for in situ cell sorting?
PL: Will show some slides... chip-based, not electrostatic, but mechanical. Working with Livermore guys

FC: Do you sort live material?
PL: Yes - sorting effects on living samples are largely unknown - work to be done . Typically pressurized to 60 psi

PL: Centrifuging technique seems pretty gentle. Acoustic tech hasn't made it into sorting applications yet.

FC: Anyone thought of breaking up cells and analyzing organelles?
PL: Done with e.g. tumor cells. Also lyse cells to extract chromosomes, then run through cytometer

PL: Can get fluorescent beads that bind to specific target - then measure beads in cytometer. Can get that fluoresce in different colors

SS: Can cells be sorted on basis of morphology?

CP: Could we sort environmental DNA?
PL: Need some kind of particle basis for cytometry
TO: Could this be a mass-spec application?
PL: Maybe

PL: No reason why a flow cytometer can't be very small - power is more challenging

PeterLopez_MBARI_09102014.pptx (application/vnd.openxmlformats-officedocument.presentationml.presentation)